3D全自动外泌体荧光检测分析系统

3D全自动外泌体荧光检测分析系统

法国Abbelight公司开发的3D全自动外泌体荧光检测分析系统是一款无需纯化的、全自动的可对单个外泌体进行表征分析的全新设备。该设备能够提供外泌体表征信息，包括外泌体粒径大小、亚型分布、携带蛋白表达、单个外泌体的膜蛋白与生物标志物共定位等。操作简单，结果可靠。

3D全自动外泌体荧光检测分析系统基于特异性免疫捕获技术，允许研究者直接分析特定群体的外泌体。通过单分子定位技术成像，可以得到单个外泌体的超分辨成像结果，尺度可以到20nm。全自动外泌体荧光检测分析系统兼容各种生物样本，除了纯化的外泌体之外，对于血液、尿液、恶性肿瘤、腹水中的外泌体也可直接检测分析，大大拓展了研究范围。

应用方向及主要特征

超高分辨单外泌体成像

左：2D单个外泌体成像；右：3D单个外泌体成像

外泌体粒径分析：通过团簇分析外泌体的整体和亚群的粒径分布

膜蛋白表征与共定位分析，以CD63，CD81&CD9为例表征其单阳，双阳和三阳的比例

外泌体内容物表征

对比穿膜处理后外泌体中内容物aGFP的表达

观测细胞中外泌体的分布情况，研究细胞中细胞器或者其他蛋白和外泌体的定位关系

应用案例

法国Abbelight公司开发的3D全自动外泌体荧光检测分析系统是一款无需纯化的、全自动的可对单个外泌体进行表征分析的全新设备。该设备能够提供单个外泌体表征信息，包括外泌体粒径大小、亚型分布、携带蛋白表达、单个外泌体的膜蛋白与生物标志物共定位等。

应用领域：肿瘤诊断，药系统开发，眼科疾病诊断，疫苗研发，脊髓受伤机制研究，血浆/血清外泌体分析，外泌体工程化，呼吸疾病诊断

测试数据

外泌体2D成像

外泌体3D成像

外泌体形态分析

发表文章

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